Cryo-EM structure of Kip3 (AMPPNP) bound to Taxol-Stabilized Microtubules
Resolution: 3.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 7rs5
Q-score: 0.235
Arellano-Santoyo H, Hernandez-Lopez RA, Stokasimov E, Wang RYR, Pellman D, Leschziner AE
bioRxiv (2021) [ DOI: doi:10.1101/2021.10.12.464151 ]
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Yeast kinesin-8/ kip3 (Complex from Saccharomyces cerevisiae)
- Taxol-stabilized microtubules (Complex from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Pig)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cryo-em structure of kip3 (amppnp) bound to taxol-stabilized microtubules (Complex)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1a chain (50 kDa, Protein from Pig)
- Yeast kinesin-8/ kip3 (39 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of Kip3 (AMPPNP) bound to GMPCPP-Stabilized Microtubules
Resolution: 4.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 7rs6
Q-score: 0.141
Arellano-Santoyo H, Hernandez-Lopez RA, Stokasimov E, Wang RY-R, Pellman D, Lesczhiner AE
bioRxiv (2024) [ DOI: doi:10.1101/2021.10.12.464151 ]
- Gmpcpp-stabilized microtubules (Complex from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Yeast kinesin-8/ kip3 (Complex from Saccharomyces cerevisiae)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Cryo-em structure of kip3 (amppnp) bound to gmpcpp-stabilized microtubules (Complex)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Kinesin-8/ kip3 (39 kDa, Protein from Saccharomyces cerevisiae)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
13pf wildtype microtubule from recombinant human tubulin decorated with kinesin
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7sj8
Q-score: 0.428
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin beta-3 chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 13pf wildtype microtubule from recombinant human tubulin and decorated with kinesin (102 kDa, Complex from Homo sapiens)
Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpo
Q-score: 0.324
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Microtubule (Complex from Sus scrofa)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Klp61f motor (Complex from Drosophila melanogaster)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Microtubule-bound klp61f motor in the amppnp state (Complex)
13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules decorated with kinesin-1 motor domain
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e7b
Q-score: 0.43
Dev Cell (2018) 47 pp. 175- [ DOI: doi:10.1016/j.devcel.2018.08.014 Pubmed: 30245156 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 13-pf 3-start gmpcpp-human alpha1b/beta3 microtubule decorated with kinesin-1 motor domain (Complex from Homo sapiens)
- Tubulin beta-3 chain (47 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
14-pf 3-start GMPCPP-human alpha1B/beta2B microtubules decorated with kinesin-1 motor domain
Resolution: 3.65 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e7c
Q-score: 0.411
Dev Cell (2018) 47 pp. 175- [ DOI: doi:10.1016/j.devcel.2018.08.014 Pubmed: 30245156 ]
- 14-pf 3-start gmpcpp-human alpha1b/beta2b microtubule decorated with kinesin-1 motor domain (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta-2b chain (47 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Homo sapiens)
S. pombe microtubule decorated with Cut7 motor domain in the AMPPNP state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mlv
Q-score: 0.355
von Loeffelholz O, Pena A, Drummond DR, Cross R, Moores CA
J Mol Biol (2019) 431 pp. 864-872 [ DOI: doi:10.1016/j.jmb.2019.01.011 Pubmed: 30659798 ]
- Alpha tubulin, beta tubulin (Complex from Schizosaccharomyces pombe 972)
- Magnesium ion (24 Da, Ligand)
- 7,11-dihydroxy-8,8,10,12,16-pentamethyl-3-[1-methyl-2-(2-methyl-thiazol-4-yl)vinyl]-4,17-dioxabicyclo[14.1.0]heptadecane-5,9-dione (507 Da, Ligand)
- Tubulin alpha-1 chain (51 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Cut7 mtd decorated s. pombe microtubule stabilized with epothilone-b (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-like protein cut7 (47 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Cut7 (Complex from Schizosaccharomyces pombe 972)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Schizosaccharomyces pombe 972h-)
KLP10A-AMPPNP in complex with curved tubulin and a microtubule
Resolution: 3.51 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0c
Q-score: 0.436
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Klp10a-amppnp motor in complex with curved tubulin and a microtubule (Complex)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein klp10a (41 kDa, Protein from Drosophila melanogaster)
- Klp10a-amppnp motor (Complex from Drosophila melanogaster)
Apo KLP10A in complex with a microtubule
Resolution: 3.78 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0i
Q-score: 0.433
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Apo-klp10a neck-motor in complex with a microtubule (Complex)
- Apo-klp10a neck-motor (Complex from Drosophila melanogaster)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
KLP10A-AMPPNP in complex with a microtubule
Resolution: 3.98 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0l
Q-score: 0.314
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Taxol (853 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
- Klp10a-amppmp in complex with a microtubule (Complex)
- Klp10a-amppmp (Complex from Drosophila melanogaster)